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Mary Queen of Scots was Catholic - nothing wrong what that you might think, but back then England's worst enemy was Spain - Catholic.
Queen Elizabeth I was 'protestant' and I do not mean just Church of England, she was both radical and non-conformist - and in the eyes of Spain, a Heretic.
Mary Queen of Scots was caught up in a plot to kill Queen Elizabeth I of England - she was held prisoner for a long time and eventually sentenced to death for treason for continuing to plot against Elizabeth while she was a prisoner.
This next link explains the threat posed by Mary Queen of Scots for Elizabeth.
http://www.historylearningsite.co.uk/mary_queen_of_scots.htm
Elizabeth I
http://www.youtube.com/watch?v=YtjKAqR_D3g&feature=related
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